Kvitova, a former semi-finalist at Roland Garros, will play world number 86 Julia Boserup of the US in the first round.
It will be her first competitive match since the Fed Cup in November and she enters the tournament with a WTA special ranking of 16 – her status at the time she last played.
Kvitova’s surgeon, Radek Kerble, described the injury she suffered as “horrific”, adding: “The chances of Petra’s hand healing well enough for her to be able to play tennis again were very low for multiple reasons.”
However, she had a racquet in her hand again by March and, after initially targeting a return at Wimbledon, brought the date forward as her recovery went better than expected.
“It was a last-minute decision – I have to start somewhere,” said Kvitova.
“Not many people believe that I can play tennis again.
“So I’m happy that I can play. I actually already won my biggest fight.
“I’m happy that I like challenges. That was one of the biggest, of course.
“So I stayed in life and I have all my fingers, I can play tennis and I can be here and be in the draw.
“Of course the hand doesn’t have that power and the strength yet, but I’m working on it. Hopefully one day everything will be perfect. But we never know still.”
Kvitova is unable to discuss the attack, which took place in her apartment in Prostejov on 20 December, because of the ongoing police investigation, but said time is helping her cope with the shock.
“I didn’t sleep well the days after, but I wasn’t really staying alone,” she said.
“I don’t really have nightmares. From the beginning I was feeling really weird when I went into the city or somewhere.
“But with time, it’s better. But of course I’m more actively watching the people around me.”
The venue was originally called Show Court One when it opened in 1988 before it was renamed in 2003 in tribute to the multiple Grand Slam winner.
The Margaret Court Arena also hosts other sporting events and concerts.
Court expressed her views in an open letter to the national airline published in The West Australian newspaper.
She said: “I am disappointed that Qantas has become an active promoter for same-sex marriage.
“I believe in marriage as a union between a man and a woman as stated in the Bible.
“Your statement leaves me no option but to use other airlines where possible for my extensive travelling.”
American singer Ryan Adams, who is playing at the arena on Friday, tweeted: “Hey Margaret Court, a PAGAN who STRONGLY SUPPORTS marriage equality (get a prenup tho) will play the arena named for you tomorrow.”
And Tom Steinfort, a senior correspondent for Nine News in Australia, tweeted: “I’ll never set foot in Margaret Court Arena again because of this.”
Tennis Australia tweeted a response: “As a legend of the sport, we respect Margaret Court’s achievements in tennis and her unmatched playing record. Her personal views are her own, and do not align with Tennis Australia’s values of equality, inclusion and diversity.”
Court won 11 Australian Open singles titles in the 1960s and 1970s, five French Opens, five US Opens and three Wimbledon crowns.